Dolgacheva L P, Galitovskaia E N, Baumuratov A S, Zinchenko V P, Bronnikov G E
Institute of Cell Biophysics RAS, Pushchino, Moscow region.
Tsitologiia. 2000;42(12):1154-8.
It has been shown that in Ca(2+)-signal generation, at the addition of norepinephrine (NE) to a suspension of freshly isolated brown preadipocytes, beta-receptors participate alongside with alpha 1-adrenoreceptors. The amplitude of cell response to 10(-5)-10(-8) M NE is approximately equal to the sum of effects of alpha 1-specific agonists (oxymetazoline or cirazoline) and beta-selective agonist isoproterenol. beta-Selective antagonist nadolol practically completely prevented the effect of NE on the intracellular Ca2+ level, while phentolamine, an antagonist of alpha-receptors, caused only a approximately 25% inhibition of the cellular response.
研究表明，在Ca(2+)信号产生过程中，向新鲜分离的棕色前脂肪细胞悬液中添加去甲肾上腺素（NE）时，β受体与α1 - 肾上腺素能受体共同发挥作用。细胞对10(-5)-10(-8) M NE的反应幅度大约等于α1特异性激动剂（羟甲唑啉或西拉唑啉）和β选择性激动剂异丙肾上腺素的作用之和。β选择性拮抗剂纳多洛尔几乎完全阻断了NE对细胞内Ca2+水平的影响，而α受体拮抗剂酚妥拉明仅使细胞反应受到约25%的抑制。
