Bocchetta M, Xiong L, Shah S, Mankin A S
Center for Pharmaceutical Biotechnology, University of Illinois, Chicago 60607, USA.
RNA. 2001 Jan;7(1):54-63. doi: 10.1017/s1355838201001650.
Interactions between tRNA or its analogs and 23S rRNA in the large ribosomal subunit were analyzed by RNA footprinting and by modification-interference selection. In the E site, tRNA protected bases G2112, A2392, and C2394 of 23S rRNA. Truncated tRNA, lacking the anticodon stem-loop, protected A2392 and C2394, but not G2112, and tRNA derivatives with a shortened 3' end protected only G2112, but not A2392 or C2394. Modification interference revealed C2394 as the only accessible nucleotide in 23S rRNA whose modification interferes with binding of tRNA in the large ribosomal subunit E site. The results suggest a direct contact between A76 of tRNA A76 and C2394 of 23S rRNA. Protections at G2112 may reflect interaction of this 23S rRNA region with the tRNA central fold.
通过RNA足迹法和修饰干扰筛选分析了tRNA或其类似物与大核糖体亚基中23S rRNA之间的相互作用。在E位点，tRNA保护23S rRNA的碱基G2112、A2392和C2394。缺少反密码子茎环的截短tRNA保护A2392和C2394，但不保护G2112，而3'端缩短的tRNA衍生物仅保护G2112，不保护A2392或C2394。修饰干扰表明C2394是23S rRNA中唯一可接近的核苷酸，其修饰会干扰tRNA在大核糖体亚基E位点的结合。结果表明tRNA的A76与23S rRNA的C2394之间存在直接接触。G2112处的保护可能反映了该23S rRNA区域与tRNA中央折叠的相互作用。
