Alibardi L
Dipartimento di Biologia Evoluzionistica Sperimentale, Università di Bologna, Italy.
Eur J Histochem. 2000;44(4):365-75.
Large neurons in the dorsal cochlear nucleus of the guinea pig which project to the inferior colliculus were identified after injections of the neural tracer WGA-HRP. Retrograde labelled cells (pyramidal and giant neurons) in the dorsal cochlear nucleus were glycine and GABA immunonegative and showed a similar ultrastructure. Between 30 and 60% of their perimeter was covered by axo-somatic boutons, most of which (>50%) contained pleomorphic synaptic vesicles. Other boutons (about 40% of total) contained flat vesicles and few (5-6%) contained round vesicles, a characteristic of the excitatory cells innervating the inferior colliculus. Immunogold-cytochemistry, coupled to silver intensification, showed that more than 50% of axo-somatic pleomorphic boutons and over 90% of boutons containing flat and pleomorphic vesicles store glycine. Rare WGA-HRP labelled axo-somatic boutons containing flat-pleomorphic vesicles were seen on pyramidal and giant neurons. This suggests that a few inhibitory collicular terminals contact the excitatory large neurons in the dorsal cochlear nucleus.
在豚鼠的背侧耳蜗核中，向中脑下丘投射的大神经元是在注射神经示踪剂WGA-HRP后被识别出来的。背侧耳蜗核中逆行标记的细胞（锥体神经元和巨型神经元）对甘氨酸和GABA免疫阴性，并且显示出相似的超微结构。它们周长的30%至60%被轴体终扣覆盖，其中大多数（>50%）含有多形性突触小泡。其他终扣（约占总数的40%）含有扁平小泡，少数（5-6%）含有圆形小泡，这是支配中脑下丘的兴奋性细胞的特征。免疫金细胞化学结合银增强显示，超过50%的轴体多形性终扣以及超过90%含有扁平小泡和多形性小泡的终扣储存甘氨酸。在锥体神经元和巨型神经元上可见罕见的标记有WGA-HRP的含有扁平-多形性小泡的轴体终扣。这表明少数抑制性下丘终末与背侧耳蜗核中的兴奋性大神经元相接触。
