Zhang G, Feng Z, Li D, Zhang H
Department of Medical Molecular Biology, Tongji Medical University, Wuhan 430030.
J Tongji Med Univ. 2000;20(3):190-3. doi: 10.1007/BF02886985.
The main features of CH50, a recombinant polypeptide of human fibronectin, activating macrophages in vivo and its anti-tumor function were investigated. After injection of CH50 and(or) transfection of IFN-gamma gene in vivo, several kinds of factors produced by macrophages were determined and the growth of tumor in vivo was measured. CH50 could enhance the production of such factors as NO, TNF and IL-1 by macrophages, but the activation of macrophages was relatively slow when CH50 was used in vivo alone. CH50 and IFN-gamma could synergistically activate macrophages rapidly in vivo no matter whether the injection of CH50 or the transfection of IFN-gamma gene was performed first. Injection of CH50 alone inhibited the formation of tumor nodes in a dose-dependent manner. Low dose of CH50 could strongly inhibit the formation of tumor nodes less than 1 mm, while high dose of CH50 could inhibit those more than 1 mm. A stronger inhibition on the growth of tumor in vivo was obtained by the synergistic effect of CH50 and IFN-gamma. CH50 and IFN-gamma, as double-signal factors for activation of macrophages, will be potentially useful in tumortherapy.
研究了人纤连蛋白重组多肽CH50在体内激活巨噬细胞的主要特征及其抗肿瘤功能。在体内注射CH50和（或）转染γ干扰素基因后，测定了巨噬细胞产生的几种因子，并检测了体内肿瘤的生长情况。CH50可增强巨噬细胞产生一氧化氮、肿瘤坏死因子和白细胞介素-1等因子，但单独在体内使用CH50时，巨噬细胞的激活相对较慢。无论先注射CH50还是先转染γ干扰素基因，CH50和γ干扰素在体内均可协同快速激活巨噬细胞。单独注射CH50可剂量依赖性地抑制肿瘤结节的形成。低剂量CH50可强烈抑制小于1毫米的肿瘤结节形成，而高剂量CH50可抑制大于1毫米的肿瘤结节。CH50和γ干扰素的协同作用对体内肿瘤生长有更强的抑制作用。CH50和γ干扰素作为激活巨噬细胞的双信号因子，在肿瘤治疗中可能具有潜在用途。
