Wang L, Xiong M, Che D, Zheng X
Department of Pathology, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030.
J Tongji Med Univ. 2000;20(3):194-6. doi: 10.1007/BF02886986.
To study the effect of c-myc antisense oligodeoxynucleotides (ODNs) on proliferation of pulmonary vascular pericytes (PC) induced by hypoxia, cell culture, dot hybridization using probe of digoxigenin-11-dUTP-labeled cDNA, 3H-thymidine incorporation, immunocytochemical technique and image analysis methods were used to observe the effect of c-myc antisense ODNs on expression of c-myc gene and proliferating cell nuclear antigen (PCNA), and 3H-thymidine incorporation of PC induced by hypoxia. The results showed that hypoxia could significantly enhance the expression of c-myc and PCNA (P < 0.01), and elevate 3H-thymidine incorporation of PC (P < 0.01), but antisense ODNs could significantly inhibit the expression of c-myc and PCNA (P < 0.05), and 3H-thymidine incorporation of PC (P < 0.01). It was suggested that hypoxia could promote the proliferation of PC by up-regulating the expression of c-myc gene, but c-myc antisense ODNs could inhibit hypoxia-induced proliferation of PC by downregulating the expression of c-myc gene.
为研究c-myc反义寡脱氧核苷酸（ODNs）对缺氧诱导的肺血管周细胞（PC）增殖的影响，采用细胞培养、地高辛-11-dUTP标记的cDNA探针斑点杂交、3H-胸腺嘧啶核苷掺入、免疫细胞化学技术及图像分析方法，观察c-myc反义ODNs对c-myc基因及增殖细胞核抗原（PCNA）表达的影响，以及对缺氧诱导的PC的3H-胸腺嘧啶核苷掺入的影响。结果显示，缺氧可显著增强c-myc和PCNA的表达（P < 0.01），并提高PC的3H-胸腺嘧啶核苷掺入（P < 0.01），但反义ODNs可显著抑制c-myc和PCNA的表达（P < 0.05），以及PC的3H-胸腺嘧啶核苷掺入（P < 0.01）。提示缺氧可通过上调c-myc基因表达促进PC增殖，而c-myc反义ODNs可通过下调c-myc基因表达抑制缺氧诱导的PC增殖。
