Nirmal B K, Rai U
Department of Zoology, University of Delhi, Delhi-110 007, India.
Indian J Exp Biol. 2000 Jul;38(7):720-6.
The total protein level in different segments of epididymis of normal lizard exhibited noticeable increase from early February to late March of a same reproductive phase. Comparison among the protein level of different epididymal segments showed insignificant variation from anterior to posterior part in early February but in late March, the protein level in posterior segment was appreciably higher than in anterior and middle segments. Further, testosterone-induced epididymal protein did not exhibit any significant quantitative variation among different regions. The electrophoretic pattern of luminal fluid from different epididymal regions of normal lizard showed 28 protein bands without any marked regional difference. However, only 16 protein bands could be demonstrated in the epididymal fluid of any region. Unlike molecular size, isoelectric focussing of testosterone induced epididymal proteins revealed that three regions of epididymis differ in their nature of protein. The number of proteins having alkaline pH range in anterior and middle regions were 4 and 3, respectively which increased upto 6 in posterior region.
正常蜥蜴附睾不同节段的总蛋白水平在同一繁殖期的2月初至3月末呈现出显著增加。不同附睾节段蛋白水平的比较显示，2月初从前部到后部变化不显著，但在3月末，后部节段的蛋白水平明显高于前部和中部节段。此外，睾酮诱导的附睾蛋白在不同区域之间未表现出任何显著的定量差异。正常蜥蜴附睾不同区域管腔液的电泳图谱显示有28条蛋白带，无明显区域差异。然而，在任何区域的附睾液中仅能显示出16条蛋白带。与分子大小不同，睾酮诱导的附睾蛋白的等电聚焦显示，附睾的三个区域在蛋白质性质上存在差异。前部和中部区域具有碱性pH范围的蛋白质数量分别为4个和3个，而后部区域增加到6个。
