Takahashi H
Department of Respiratory Oncology and Molecular Medicine, Division of Cancer Control, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575.
Rinsho Byori. 2000 Dec;48(12):1130-5.
In Japan, molecular diagnostic techniques and some other diagnostic tools for respiratory infections have been clinically introduced and their use has spread in recent years. In particular, PCR is now commonly used even at the clinical microbiology laboratory level, because PCR based direct detection of infectious disease from various clinical specimens has some advantages over other diagnostic methods. Since PCR is a highly sensitive, specific, rapid method and suitable for slow-growing or culture-difficult pathogens, we must take care in regard to the risk of contamination, the possibility of false-negative results by various PCR inhibitors in the clinical sample, or unstable sensitivity easily influenced by the quality of samples and DNA extraction step.
在日本，分子诊断技术和其他一些用于呼吸道感染的诊断工具已在临床上得到应用，并且近年来其使用范围不断扩大。特别是，如今即使在临床微生物学实验室层面，PCR也已普遍使用，因为基于PCR从各种临床标本中直接检测传染病具有一些优于其他诊断方法的优势。由于PCR是一种高度灵敏、特异、快速的方法，适用于生长缓慢或难以培养的病原体，我们必须注意污染风险、临床样本中各种PCR抑制剂导致假阴性结果的可能性，或易受样本质量和DNA提取步骤影响的不稳定敏感性。
