日本鼩鼱（Urotrichus talpoides）生精上皮的周期。

The cycle of the seminiferous epithelium in the greater Japanese shrew mole, Urotrichus talpoides.

作者信息

Mizukam T, Kuwahara S, Ohmura M, Iinuma Y, Izumikubo J, Hagiwara M, Kurohmaru M, Hayashi Y, Nishida T

机构信息

Laboratory of Anatomy and Physiology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.

出版信息

J Vet Med Sci. 2001 Jan;63(1):31-5. doi: 10.1292/jvms.63.31.

DOI:10.1292/jvms.63.31

PMID:11217059

Abstract

Spermatogenesis and acrosomal formation in the greater Japanese shrew mole, Urotrichus talpoides, were studied by light microscopy. On the basis of acrosomal changes, morphology of spermatid head, nuclear shape, appearance of meiotic figures, location of spermatid and period of spermiation, the cycle of the seminiferous epithelium was classified into 12 stages, and developing spermatids could be divided into 15 steps. The mean relative frequencies of stages from I to XII were 10.9, 8.7, 9.8, 7.3, 8.5, 10.3, 12.5, 8.7, 5.8, 5.4, 5.1 and 7.1%, respectively. Similar to the case in the musk shrew, the spermatid nucleus of the greater Japanese shrew mole remained in the middle region of the seminiferous epithelium and only the acrosome extended towards the basement membrane. The elongation of the acrosome, however, was not prominent. The proacrosomal vesicle first appeared in stage II and then one large and round granule was seen in stage III. The acrosomal vesicle became flattened on the surface of the nucleus in stage IV. Spreading of the acrosomic system has been recognized from stage VII. In stage VII, spermiation occurred. In stage IX, the spermatid nucleus began to elongate. Elongation and condensation of the nucleus were clearly observed in stage X. In stage XII, pachytene spermatocytes divided into diplotene spermatocytes. In stage XII, meiotic figures and secondary spermatocytes were observed.

摘要

利用光学显微镜对日本大鼩鼱（Urotrichus talpoides）的精子发生和顶体形成进行了研究。根据顶体变化、精子细胞头部形态、核形状、减数分裂图像的出现、精子细胞的位置和精子释放期，将生精上皮的周期分为12个阶段，发育中的精子细胞可分为15个步骤。从I到XII阶段的平均相对频率分别为10.9%、8.7%、9.8%、7.3%、8.5%、10.3%、12.5%、8.7%、5.8%、5.4%、5.1%和7.1%。与麝鼩的情况类似，日本大鼩鼱的精子细胞核留在生精上皮的中部区域，只有顶体向基底膜延伸。然而，顶体的伸长并不明显。前顶体泡首先出现在II期，然后在III期可见一个大的圆形颗粒。顶体泡在IV期在细胞核表面变平。从VII期开始可观察到顶体系统的扩展。在VII期发生精子释放。在IX期，精子细胞核开始伸长。在X期可清楚观察到细胞核的伸长和浓缩。在XII期，粗线期精母细胞分裂为双线期精母细胞。在XII期，观察到减数分裂图像和次级精母细胞。