Terada T, Sakagami R, Tabuchi Y, Maeda M
Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka UCniversity, Suita, Japan.
Biol Pharm Bull. 2001 Feb;24(2):135-9. doi: 10.1248/bpb.24.135.
Trefoil peptides (TFFs) with a unique trefoil domain(s) are presumed to function in protection and repair of the gastrointestinal epithelial layer. Three peptide family members are differently distributed in the mouse gastrointestinal tract: TFF1/pS2 specifically in stomach, TFF2/SP mainly in stomach, pancreas and duodenum, and TFF3/ITF in intestine. We cloned and sequenced the mouse TFF1 gene 5'-upstream region by means of the genomic walking procedure. The cloned region was ligated to the luciferase reporter gene and then introduced into mouse gastric surface mucous GSM10 cells which express TFF1 and TFF2. The minimum promoter was located in the region containing the TATA-box between -39 and the transcriptional start site. Further upstream regions stimulated (-2192-- -1630bp, -641-- -243bp, -137-- -39bp) and inhibited (-1630-- -641bp, -243-- -137 bp) luciferase gene expression. These regions as well as short segments conserved in the mouse and human 5'-upstream sequences may be important for modulation of the mRNA level of the TFF1 gene.
具有独特三叶结构域的三叶肽（TFFs）被认为在胃肠道上皮层的保护和修复中发挥作用。三个肽家族成员在小鼠胃肠道中的分布不同：TFF1/pS2特异性分布于胃，TFF2/SP主要分布于胃、胰腺和十二指肠，TFF3/ITF分布于肠道。我们通过基因组步移法克隆并测序了小鼠TFF1基因的5'-上游区域。将克隆区域连接到荧光素酶报告基因上，然后导入表达TFF1和TFF2的小鼠胃表面黏液GSM10细胞中。最小启动子位于包含TATA框的-39至转录起始位点之间的区域。进一步的上游区域刺激（-2192-- -1630bp、-641-- -243bp、-137-- -39bp）和抑制（-1630-- -641bp、-243-- -137bp）荧光素酶基因表达。这些区域以及小鼠和人类5'-上游序列中保守的短片段可能对调节TFF1基因的mRNA水平很重要。
