Soule J D, Kudrna D A, Kleinhofs A
Department of Genetics and Cell Biology, Washington State University, Pullman 99165-6420, USA.
J Hered. 2000 Nov-Dec;91(6):483-7. doi: 10.1093/jhered/91.6.483.
Mutations in homeotic genes disturb the spatial and temporal patterns of development, often leading to the appearance of tissues in abnormal locations. Many homeotic genes, involved in flower development, code for proteins with a highly conserved domain called the MADS box, which acts as a sequence-specific DNA binding protein. Two floral development mutants were isolated from a fast neutron irradiated M2 barley population. The phenotypes are multiovary, that is, stamens replaced with carpels, designated mo7a, and stamens replaced with carpels and lodicules converted to leaflike structures, designated mo6b. These phenotypes resemble the Arabidopsis mutants APETALA3 (AP3) and PISTILATA (PI). The mo6b and mo7a mutants were mapped to the centromeric region of chromosome 1 (7H) and to the telomeric region of chromosome 3 (3H), respectively.
同源异型基因的突变会扰乱发育的时空模式，常常导致组织在异常位置出现。许多参与花发育的同源异型基因编码具有一个名为MADS框的高度保守结构域的蛋白质，该结构域作为一种序列特异性DNA结合蛋白发挥作用。从经快中子辐照的M2大麦群体中分离出两个花发育突变体。其表型为多子房，即雄蕊被心皮取代，命名为mo7a，以及雄蕊被心皮取代且浆片转化为叶状结构，命名为mo6b。这些表型类似于拟南芥突变体APETALA3（AP3）和PISTILATA（PI）。mo6b和mo7a突变体分别被定位到染色体1（7H）的着丝粒区域和染色体3（3H）的端粒区域。
