Verghese S L, Padmaja P, Sutha P, Mathew T, John E S
Department of Microbiology, Institute of Cardio-Vascular Diseases, Chennai.
Indian J Pathol Microbiol. 2000 Jul;43(3):343-6.
Although extremely rare 10 years ago, antifungal drug resistance is becoming a major problem in certain populations, especially in those infected with HIV. This study was undertaken to study the resistance of Candida species isolated in our hospital to Fluconazole using Chrom agar Candida. The Candida strains which were routinely isolated from clinical specimens like blood, urine, sputum, pus, fluid and homograft isolates were included in the study. 142 Candida isolates were tested by using Chrom agar Candida incorporated with fluconazole. 16 strains were found to be resistant to Fluconazole and 126 strains sensitive to Fluconazole. Nine were C tropicalis, 3 C krusei, 2 C guillermondii, 1 Geotrichum candidum and one was an unidentified strain of Candida. The MIC of the 16 strains were done using RPMI 1640 medium by macro broth dilution method. MIC of 9 strains was 64 & > 64 micrograms/ml of 6 strains 32 micrograms/ml and 1 strain 16 micrograms/ml.
尽管抗真菌药物耐药性在10年前极为罕见，但现在正成为某些人群中的一个主要问题，尤其是在感染了艾滋病毒的人群中。本研究旨在使用念珠菌显色培养基研究我院分离的念珠菌属对氟康唑的耐药性。本研究纳入了常规从血液、尿液、痰液、脓液、体液和同种异体移植分离物等临床标本中分离出的念珠菌菌株。通过使用含氟康唑的念珠菌显色培养基对142株念珠菌分离株进行了检测。发现16株对氟康唑耐药，126株对氟康唑敏感。其中9株为热带念珠菌，3株为克柔念珠菌，2株为季也蒙念珠菌，1株为白吉利丝孢酵母，1株为未鉴定的念珠菌菌株。采用RPMI 1640培养基通过常量肉汤稀释法测定了16株菌株的最低抑菌浓度（MIC）。9株菌株的MIC为64及＞64微克/毫升，6株为32微克/毫升，1株为16微克/毫升。
