Hu W, Hasebe K, Tanaka K, Inoue S, Nagai M
Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo-060-0810, Japan.
Analyst. 2000 Dec;125(12):2160-2. doi: 10.1039/b007147l.
The hydronium ion (H3O+), the most essential species to every field of chemistry, was successfully separated from other cations. This major goal was achieved by a HPLC approach in which a reversed-phase ODS which had been initially modified with dodecylsulfate was used as the stationary phase and an aqueous KNO3 solution was used as the mobile phase. Of all tested monovalent cations (analyte ions), the hydronium ion (H3O+) showed the highest affinity towards the stationary phase, followed by NH4+, Na+, and then Li+. The hydronium ion, after the separation, was directly detected using conductivity and the detection signal (conductivity) was in linear proportion (linear range 7.8 x 10(-4)-0.45 M; injection volume, 100 microL) to the concentration of the acid species of the tested sample. This proposed HPLC method was applicable to separate/detect H3O+ from both strong and weak acidic samples.
水合氢离子（H3O+）是化学各个领域中最重要的物种，已成功地与其他阳离子分离。这一主要目标是通过一种高效液相色谱（HPLC）方法实现的，该方法中使用最初用十二烷基硫酸盐改性的反相ODS作为固定相，用硝酸钾水溶液作为流动相。在所有测试的单价阳离子（分析物离子）中，水合氢离子（H3O+）对固定相表现出最高的亲和力，其次是NH4+、Na+，然后是Li+。分离后的水合氢离子通过电导率直接检测，检测信号（电导率）与测试样品中酸性物质的浓度呈线性比例关系（线性范围为7.8×10(-4)-0.45 M；进样体积为100微升）。所提出的HPLC方法适用于从强酸性和弱酸性样品中分离/检测H3O+。
