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通过单向放射免疫扩散法对胎盘磷酸酶抗体进行免疫定量;抗体亲和力和免疫方案的影响

Immunoquantitation of antibody to placental phosphatase by single radial immunodiffusion; effect of antibody avidity and immunization protocol.

作者信息

Doellgast G J

出版信息

J Immunol Methods. 1979;27(3):257-62. doi: 10.1016/0022-1759(79)90222-9.

Abstract

The radial immunodiffusion method was used to titer antisera to human placental alkaline phosphatase. When this was done with diluted enzyme and antisera, it was found that early-course antisera consistently yielded low titers. This observation was consistent with the known low avidity of such antisera.

摘要

采用放射免疫扩散法测定抗人胎盘碱性磷酸酶抗血清的效价。当用稀释的酶和抗血清进行此项操作时,发现早期抗血清的效价始终较低。这一观察结果与这类抗血清已知的低亲和力相符。

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