由P1噬菌体“成瘾模块”phd-doc介导的分离后杀伤作用需要大肠杆菌程序性细胞死亡系统mazEF。
Postsegregational killing mediated by the P1 phage "addiction module" phd-doc requires the Escherichia coli programmed cell death system mazEF.
作者信息
Hazan R, Sat B, Reches M, Engelberg-Kulka H
机构信息
Department of Molecular Biology, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
出版信息
J Bacteriol. 2001 Mar;183(6):2046-50. doi: 10.1128/JB.183.6.2046-2050.2001.
Abstract
"Addiction modules" consist of two genes; the product of the second is long lived and toxic, while the product of the first is short lived and antagonizes the lethal action of the toxin. The extrachromosomal addiction module phd-doc, located on the P1 prophage, is responsible for the postsegregational killing effect (death of plasmid-free cells). The Escherichia coli chromosomal addiction module analogue, mazEF, is responsible for the induction of programmed cell death. Here we show that the postsegregational killing mediated by the P1 phd-doc module depends on the presence of the E. coli mazEF system. In addition, we demonstrate that under conditions of postsegregational killing, mediated by phd-doc, protein synthesis of E. coli is inhibited. Based on our findings, we suggest the existence of a coupling between the phd-doc and mazEF systems.
摘要
“成瘾模块”由两个基因组成；第二个基因的产物寿命长且具有毒性，而第一个基因的产物寿命短且能拮抗毒素的致死作用。位于P1原噬菌体上的染色体外成瘾模块phd-doc负责后分离杀伤效应（无质粒细胞的死亡）。大肠杆菌染色体成瘾模块类似物mazEF负责程序性细胞死亡的诱导。在这里我们表明，由P1 phd-doc模块介导的后分离杀伤取决于大肠杆菌mazEF系统的存在。此外，我们证明在由phd-doc介导的后分离杀伤条件下，大肠杆菌的蛋白质合成受到抑制。基于我们的发现，我们推测phd-doc和mazEF系统之间存在耦合。
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本文引用的文献
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Programmed cell death in Escherichia coli: some antibiotics can trigger mazEF lethality.大肠杆菌中的程序性细胞死亡：某些抗生素可引发mazEF致死效应。
J Bacteriol. 2001 Mar;183(6):2041-5. doi: 10.1128/JB.183.6.2041-2045.2001.
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Addiction modules and programmed cell death and antideath in bacterial cultures.细菌培养中的成瘾模块、程序性细胞死亡和抗死亡
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An Escherichia coli chromosomal "addiction module" regulated by guanosine [corrected] 3',5'-bispyrophosphate: a model for programmed bacterial cell death.一种受鸟苷[校正后]3',5'-双焦磷酸调控的大肠杆菌染色体“成瘾模块”：一种程序性细菌细胞死亡的模型。
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Plasmid addiction genes of bacteriophage P1: doc, which causes cell death on curing of prophage, and phd, which prevents host death when prophage is retained.噬菌体P1的质粒成瘾基因：doc，在原噬菌体治愈时导致细胞死亡；以及phd，在原噬菌体保留时防止宿主死亡。
J Mol Biol. 1993 Oct 5;233(3):414-28. doi: 10.1006/jmbi.1993.1521.
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Programmed cell death in bacterial populations.细菌群体中的程序性细胞死亡。
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Addiction protein Phd of plasmid prophage P1 is a substrate of the ClpXP serine protease of Escherichia coli.质粒原噬菌体P1的成瘾蛋白Phd是大肠杆菌ClpXP丝氨酸蛋白酶的一个底物。
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3274-7. doi: 10.1073/pnas.92.8.3274.
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