几种物种的α-乳白蛋白的分离、组成及功能特性

Isolation, composition and functional properties of alpha-lactalbumins from several species.

Quarfoth G J, Jenness R

Biochim Biophys Acta. 1975 Feb 27;379(2):476-87. doi: 10.1016/0005-2795(75)90154-3.

Abstract

Galactosyltranferases were isolated from bovine and pig milk and alpha-labtalbumins from milks of cow, pig, dog, rabbit, baboon and chimpanzee. 2. Alpha-Lactalbumins were characterized by gel electrophoresis, amino acid composition, ultraviolet absorbance, and ability to promote synthesis of lactose by the galactosyltransferases. 3. The order of K-m values of the various alpha-lactalbumins was the same with both cow and pig galactosyltransferases and differed from the order predicted from taxonomical classification of the species. 4. A280/A260 abosrbance ratios were correlated with K-m values suggesting involvement of tyrosyl groups in the function of alpha-lactalbumin.

摘要

从牛乳和猪乳中分离出半乳糖基转移酶，并从牛、猪、狗、兔、狒狒和黑猩猩的乳汁中分离出α-乳白蛋白。2. 通过凝胶电泳、氨基酸组成、紫外线吸收以及促进半乳糖基转移酶合成乳糖的能力对α-乳白蛋白进行了表征。3. 各种α-乳白蛋白的K-m值顺序对于牛和猪的半乳糖基转移酶来说是相同的，并且与根据物种分类学预测的顺序不同。4. A280/A260吸光度比值与K-m值相关，表明酪氨酸基团参与了α-乳白蛋白的功能。