Hitomi M, Stacey D W
Department of Molecular Biology, The Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
FEBS Lett. 2001 Feb 16;490(3):123-31. doi: 10.1016/s0014-5793(01)02115-9.
Synchronization used to study cell cycle progression may change the characteristics of rapidly proliferating cells. By combining time-lapse, quantitative fluorescent microscopy and microinjection, we have established a method to analyze the cell cycle progression of individual cells without synchronization. This new approach revealed that rapidly growing NIH3T3 cells make a Ras-dependent commitment for completion of the next cell cycle while they are in G2 phase of the preceding cell cycle. Thus, Ras activity during G2 phase induces cyclin D1 expression. This expression continues through the next G1 phase even in the absence of Ras activity, and drives cells into S phase.
用于研究细胞周期进程的同步化可能会改变快速增殖细胞的特性。通过结合延时、定量荧光显微镜和显微注射技术，我们建立了一种无需同步化就能分析单个细胞周期进程的方法。这种新方法揭示，快速生长的NIH3T3细胞在前一个细胞周期的G2期时就对下一个细胞周期的完成做出了依赖Ras的承诺。因此，G2期的Ras活性诱导细胞周期蛋白D1表达。即使在没有Ras活性的情况下，这种表达也会持续到下一个G1期，并驱动细胞进入S期。
