Nakamura Y, Okuno S, Kitani T, Otake K, Sato F, Fujisawa H
Section of Neuroanatomy, Graduate School of Medical and Dental Research, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, 113-8519, Tokyo, Japan.
Neurosci Res. 2001 Feb;39(2):175-88. doi: 10.1016/s0168-0102(00)00209-1.
We examined regional and intracellular distribution of Ca(2+)/calmodulin-dependent protein kinase kinase beta (CaM-KK beta), which activated Ca(2+)/calmodulin-dependent protein kinase I and IV (CaM-K I and IV) immunohistochemically in the central nervous system of the rat by light and electron microscopy. Although most neurons in the brain and spinal cord exhibited the immunoreactivity, no labeled neurons were observed in the globus pallidus or entopeduncular nucleus, and only a small number of neurons showed weak immunoreactivity in the substantia nigra pars reticulata. In general, the immunoreactivity was observed both in the cytoplasm and cellular nucleus, although the immunoreactivity was not found in the cellular nucleus in some large neurons such as in the mesencephalic trigeminal nucleus, lateral vestibular nucleus or gigant cellular reticular formation. As to motoneurons of the cranial nerve nuclei and the anterior horn of the spinal cord, they revealed the immunoreactivity both in the cytoplasm and nucleus. The reaction product appeared as fine granules in the cytoplasm and nucleus under light microscopy. Electron microscopic observations confirmed that the reaction product was localized mainly on the Golgi apparatus or on the nuclear chromatin. Immunolabeling for antibody against CaM-KK beta was discussed with the distribution of CaM-K I, IV and another CaM-KK, CaM-KK alpha, in the central nervous system.
我们通过光学显微镜和电子显微镜，免疫组织化学地研究了大鼠中枢神经系统中钙/钙调蛋白依赖性蛋白激酶激酶β（CaM-KKβ）的区域和细胞内分布，该激酶可激活钙/钙调蛋白依赖性蛋白激酶I和IV（CaM-K I和IV）。尽管脑和脊髓中的大多数神经元都表现出免疫反应性，但在苍白球或内囊核中未观察到标记的神经元，并且在黑质网状部中只有少数神经元显示出弱免疫反应性。一般来说，免疫反应性在细胞质和细胞核中均有观察到，尽管在一些大神经元如中脑三叉神经核、外侧前庭核或巨细胞网状结构的细胞核中未发现免疫反应性。至于脑神经核和脊髓前角的运动神经元，它们在细胞质和细胞核中均显示出免疫反应性。在光学显微镜下，反应产物在细胞质和细胞核中呈细颗粒状。电子显微镜观察证实，反应产物主要定位于高尔基体或核染色质上。本文还讨论了抗CaM-KKβ抗体的免疫标记与中枢神经系统中CaM-K I、IV以及另一种CaM-KK即CaM-KKα的分布情况。
