Fachbereich Biologie, Universität Konstanz, 78457 Konstanz, Germany.
Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):448-50. doi: 10.1107/s0907444901000397.
A periplasmic binding protein (ProX) for the compatible solutes glycine betaine and proline betaine from Escherichia coli was crystallized using the hanging-drop vapour-diffusion method. Crystals were grown using a protein concentration of 10 mg ml(-1) and a precipitant of 26-28% PEG 4000 in 50 mM PIPES pH 6.2-6.4. Native diffraction data to 1.93 A resolution have been obtained from crystals at 290 K. The crystals belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 48.1, b = 55.0, c = 115.7 A, and contain one molecule per asymmetric unit.
利用悬滴气相扩散法，使来自大肠杆菌的用于相容性溶质甘氨酸甜菜碱和脯氨酸甜菜碱的周质结合蛋白（ProX）结晶。使用浓度为10 mg/ml的蛋白质和50 mM PIPES pH 6.2 - 6.4中26 - 28%的PEG 4000作为沉淀剂来生长晶体。在290 K下从晶体获得了分辨率为1.93 Å的天然衍射数据。晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 48.1、b = 55.0、c = 115.7 Å，每个不对称单元包含一个分子。
