Shindo-Okada N, Takeuchi K, Nagamachi Y
Biology Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan.
Jpn J Cancer Res. 2001 Feb;92(2):174-83. doi: 10.1111/j.1349-7006.2001.tb01080.x.
This article reports the establishment of variant cell lines with high and low metastatic potential by repeated selection and the dilution plating technique. Five clones with high metastatic potential, Lu-2, Lu-7, Lu-4, Lu-1 and Lu-5, and four clones with low metastatic potential, 3S, 7S, 8S and 13S, were established from PC-14 human lung adenocarcinoma. The high-metastatic cell lines produced enhanced lung metastases, but the low-metastatic cell lines did not produce lung metastasis by injection into the tail vein of 5-week-old BALB / c nude mice. The high-metastatic cell lines produced enhanced tumors on both visceral and parietal pleurae, and enhanced metastases to the mediastinum and contralateral pleural cavity. The low-metastatic cell lines produced reduced tumors on both visceral and parietal pleurae and reduced metastases to the mediastinum and contralateral pleural cavity after injection into the left preceral cavity of the nude mice. When the nine variant cell lines and original PC-14 cells were embedded in collagen gels, the PC-14 cells and the low-metastatic cell lines gave rise to colonies with a dendritic morphology, and cells were tightly associated. The high-metastatic cell lines were more loosely associated and scattered into three-dimensional colonies. These nine cloned cell lines originated from heterogeneous populations of the parental PC-14 cells should be useful tools for studying the process of metastasis of lung cancer.
本文报道了通过反复筛选和稀释平板技术建立具有高转移潜能和低转移潜能的变异细胞系。从人肺腺癌PC-14细胞系中建立了5个具有高转移潜能的克隆，即Lu-2、Lu-7、Lu-4、Lu-1和Lu-5，以及4个具有低转移潜能的克隆，即3S、7S、8S和13S。将这些高转移细胞系注射到5周龄BALB/c裸鼠的尾静脉中，可增强肺转移，而低转移细胞系则不会产生肺转移。高转移细胞系在内脏和壁层胸膜上均产生增强的肿瘤，并增强向纵隔和对侧胸腔的转移。将低转移细胞系注射到裸鼠的左前胸腔后，在内脏和壁层胸膜上产生的肿瘤减少，向纵隔和对侧胸腔的转移也减少。当将这9个变异细胞系和原始的PC-14细胞包埋在胶原凝胶中时，PC-14细胞和低转移细胞系形成具有树突状形态的集落，细胞紧密相连。高转移细胞系联系较松散，分散形成三维集落。这些源自亲代PC-14细胞异质群体的9个克隆细胞系应是研究肺癌转移过程的有用工具。
