Zakhartchouk A N, Godson D L, Babiuk L A, Tikoo S K
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Intervirology. 2001;44(1):29-35. doi: 10.1159/000050027.
Based on the DNA sequence and known mRNA structures, the early region 3 (E3) of bovine adenovirus (BAV)-3 has the potential to encode four proteins. One of them (121R) is produced as a 14.5-kD protein throughout infection. Analysis of the 121R protein showed limited homology to a 14.7K protein of human adenovirus (HAV)-5. Interestingly, both anti-14.7K and anti-121R sera immunoprecipitated a 14.5-kD protein from cells infected with BAV-3. To determine if 121R is functionally related to 14.7K, we constructed a recombinant E3-deleted HAV-5 (AdKV121) expressing the BAV-3 E3 121R protein. Mouse C3HA cells infected with HAV-5 mutant dl 758 (deletion of 14.7K) were sensitive to TNF lysis. However, wild-type HAV-5- or recombinant AdKV121-infected cells were resistant to TNF-induced cytolysis. Our result show that the BAV-3 E3 121R protein is serologically and functionally related to the 14.7K protein encoded by the E3 region of HAV-5.
根据DNA序列和已知的mRNA结构，牛腺病毒（BAV）-3的早期区域3（E3）有潜力编码四种蛋白质。其中一种（121R）在整个感染过程中产生一种14.5-kD的蛋白质。对121R蛋白的分析显示，它与人类腺病毒（HAV）-5的一种14.7K蛋白有有限的同源性。有趣的是，抗14.7K和抗121R血清都能从感染BAV-3的细胞中免疫沉淀出一种14.5-kD的蛋白质。为了确定121R是否在功能上与14.7K相关，我们构建了一种表达BAV-3 E3 121R蛋白的重组E3缺失型HAV-5（AdKV121）。感染HAV-5突变体dl 758（缺失14.7K）的小鼠C3HA细胞对TNF裂解敏感。然而，感染野生型HAV-5或重组AdKV121的细胞对TNF诱导的细胞溶解具有抗性。我们的结果表明，BAV-3 E3 121R蛋白在血清学和功能上与HAV-5 E3区域编码的14.7K蛋白相关。
