Stenkamp D L, Powers M K, Carney L H, Cameron D A
Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844-3051, USA.
J Comp Neurol. 2001 Mar 19;431(4):363-81. doi: 10.1002/1096-9861(20010319)431:4<363::aid-cne1076>3.0.co;2-7.
After its destruction by intraocular injection of ouabain, the goldfish retina regenerates, but little is known about the histogenesis of the new tissue, including the structure and formation of regenerated cell mosaic patterns. In an effort to determine how retinal cells are generated and spatially organized within retina regenerated after ouabain injection, in situ hybridization and immunocytochemical techniques were combined with computational analyses of two-dimensional spatial patterns of identified neurons. Labeling with specific opsin riboprobes revealed two distinct cone patterns in the ouabain-injected eyes, each of which was different from the relatively orderly cone patterns of native retina. Central, regenerated regions had sparse aggregates of cones, and a relatively lower density of each cone type. Peripheral regions of experimental retina, likely derived from the circumferential germinal zone, had high densities of all cone types, each of which tended to be distributed randomly. The spatial patterns of inner retinal neurons in experimental eyes were also disorganized with respect to native retina. These results indicate that although some aspects of retinal regeneration resemble normal retinal development and growth, ouabain-induced regeneration does not produce well-organized mosaics of neurons, indicating a failure of the developmental interactions needed for proper pattern formation, which in turn could compromise visual recovery. Furthermore, the distinct cone patterns in different regions of experimental retina support the hypothesis that new goldfish retina arises via two spatially and cellularly distinct mechanisms after exposure to ouabain.
在用哇巴因进行眼内注射破坏其结构后，金鱼视网膜会再生，但对于新组织的组织发生过程，包括再生细胞镶嵌模式的结构和形成，人们了解甚少。为了确定在哇巴因注射后再生的视网膜内视网膜细胞是如何产生和在空间上组织起来的，将原位杂交和免疫细胞化学技术与对已识别神经元的二维空间模式的计算分析相结合。用特定的视蛋白核糖探针进行标记显示，在注射哇巴因的眼睛中有两种不同的视锥细胞模式，每种模式都与天然视网膜中相对有序的视锥细胞模式不同。中央再生区域视锥细胞聚集稀疏，每种视锥细胞类型的密度相对较低。实验视网膜的周边区域可能源自周边生发区，所有视锥细胞类型的密度都很高，且每种视锥细胞类型往往随机分布。实验眼中视网膜内层神经元的空间模式相对于天然视网膜也杂乱无章。这些结果表明，尽管视网膜再生的某些方面类似于正常的视网膜发育和生长，但哇巴因诱导的再生并不能产生组织良好的神经元镶嵌结构，这表明正常模式形成所需的发育相互作用失败，进而可能影响视觉恢复。此外，实验视网膜不同区域不同的视锥细胞模式支持了这样一种假说，即金鱼视网膜在接触哇巴因后通过两种在空间和细胞层面上不同的机制产生。
