Zemplenyi T, Blankenhorn D H, Rosenstein A J
Circ Res. 1975 May;36(5):640-6. doi: 10.1161/01.res.36.5.640.
The activity of lipoamide dehydorgenase (E.C.1.6.4.3) was measured in arterial homogenates from very young pigeons (5-8 weeks old) known to differ in their susceptibility to atherosclerosis. The activity of the arterial enzyme was significantly lower in the atherosclerosis-susceptible White Carneau pigeons than it was in the atherosclerosis-resistant Show Racer pigeons. Lipoamide dehydrogenase is a component of the pyruvate dehydrogenase and alpha-ketoglutarate multienzyme complexes. The first complex catalyzes the conversion of pyruvate to oxaloacetate via acetyl-CoA, and this reaction represents a crucial link between glycolysis and the Krebs cycle. The second complex is essential for the oxidative breakdown of carbohydrates, fats, and amino acids via the Krebs cycle. Reduced activity of these complexes, resulting from low activity of lipoamide dehydrogenase, favors reduction of pyruvate to lactate and a shift to glycolysis. This situation is in accord with other results obtained in avian and human arteries which appear to indicate a higher rate of glycolysis in atherosclerosis-susceptible and atherosclerotic arteries. It appears that the increased dependence of the White Carneau arteries on glycolysis, suggested by the reduced lipoamide dehydrogenase activity, facilitates the development of atherosclerosis in this pigeon strain.
在已知对动脉粥样硬化易感性不同的幼鸽（5 - 8周龄）的动脉匀浆中，测定了硫辛酰胺脱氢酶（E.C.1.6.4.3）的活性。在易患动脉粥样硬化的白卡诺鸽中，动脉酶的活性显著低于抗动脉粥样硬化的赛鸽。硫辛酰胺脱氢酶是丙酮酸脱氢酶和α - 酮戊二酸多酶复合体的一个组成部分。第一个复合体催化丙酮酸通过乙酰辅酶A转化为草酰乙酸，并且该反应代表糖酵解和三羧酸循环之间的关键联系。第二个复合体对于通过三羧酸循环对碳水化合物、脂肪和氨基酸的氧化分解至关重要。由于硫辛酰胺脱氢酶活性低而导致这些复合体的活性降低，有利于丙酮酸还原为乳酸并转向糖酵解。这种情况与在禽类和人类动脉中获得的其他结果一致，这些结果似乎表明在易患动脉粥样硬化和动脉粥样硬化的动脉中糖酵解速率更高。似乎硫辛酰胺脱氢酶活性降低所表明的白卡诺动脉对糖酵解的依赖性增加，促进了该品系鸽子动脉粥样硬化的发展。
