Ramaraj P, Rao A J
Department of Diabetes, Endocrinology & Metabolism, The University of Hope National Medical Centre, Duarte, CA 91007, USA.
Asian J Androl. 1999 Dec;1(4):191-4.
To demonstrate the role of the 8 kDa peptide in regulation of testosterone production by rat Leydig cells.
A peptide similar to 8 kDa peptide purified from immature rat Leydig cells was isolated and purified from rat lung cytosol. Immunological and structural similarity between the peptides purified from lung and Leydig cells was established by Western blot and tryptic map comparison respectively.
Addition of the 8 kDa peptide 10, 50, 100, and 150 microg decreased the production of testosterone in Leydig cells dose-dependently. But the addition of the peptide 150 microg along with hCG had no effect on hCG-stimulated increase in testosterone production.
In vitro addition of the peptide purified from lung cytosol to adult rat Leydig cells resulted in a concentration-dependent decrease in basal testosterone production although it had no effect on hCG-stimulated testosterone production.
证明8 kDa肽在调节大鼠睾丸间质细胞睾酮分泌中的作用。
从大鼠肺胞质溶胶中分离并纯化出一种与从未成熟大鼠睾丸间质细胞中纯化的8 kDa肽相似的肽。分别通过蛋白质免疫印迹法和胰蛋白酶图谱比较法确定从肺和睾丸间质细胞中纯化出的肽之间的免疫和结构相似性。
添加10、50、100和150微克的8 kDa肽可使睾丸间质细胞中的睾酮分泌呈剂量依赖性减少。但添加150微克该肽与hCG一起对hCG刺激的睾酮分泌增加没有影响。
体外将从肺胞质溶胶中纯化的肽添加到成年大鼠睾丸间质细胞中，导致基础睾酮分泌呈浓度依赖性降低，尽管它对hCG刺激的睾酮分泌没有影响。
