胃蛋白酶衍生的牛血红蛋白片段的抗菌活性

Antibacterial activity of a pepsin-derived bovine hemoglobin fragment.

作者信息

Froidevaux R, Krier F, Nedjar-Arroume N, Vercaigne-Marko D, Kosciarz E, Ruckebusch C, Dhulster P, Guillochon D

机构信息

Laboratoire de Technologie des Substances Naturelles, Institut Universitaire de Technologie, Département Génie Biologique, Université des Sciences et Technologie de Lille, PO Box 179, 59653 Villeneuve d'Ascq Cedex, France.

出版信息

FEBS Lett. 2001 Feb 23;491(1-2):159-63. doi: 10.1016/s0014-5793(01)02171-8.

DOI:10.1016/s0014-5793(01)02171-8

PMID:11226440

Abstract

Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fragment of the alpha chain of hemoglobin. The minimum inhibitory concentration and mode of action of this peptide towards Micrococcus luteus strain A270 were determined. Hemolytic assay, interaction with liposomes, and study of its structure in solution were also performed.

摘要

牛血红蛋白的胃蛋白酶消化产生一种具有抗菌活性的片段。该肽通过两步法纯化至同质，包括阴离子交换色谱和制备型反相高效液相色谱。质量测定和片段分析表明，该肽对应于血红蛋白α链的1-23片段。测定了该肽对藤黄微球菌A270菌株的最小抑菌浓度和作用方式。还进行了溶血试验、与脂质体的相互作用以及其在溶液中的结构研究。