Freiberg B, Höhlich B, Haas B, Saalmüller A, Pfaff E, Marquardt O
Bundesforschungsanstalt für Viruskrankheiten der Tiere, Paul-Ehrlich-Strasse 28, D-72076, Tübingen, Germany.
J Virol Methods. 2001 Apr;92(2):199-205. doi: 10.1016/s0166-0934(00)00287-1.
The characterization of monoclonal antibodies raised against the foot-and-mouth disease virus isolates A22 Iraq/1964, Asia1 Shamir-Israel/1989, and SAT1 Zimbabwe/1989 with regard to neutralizing activity and sensitivity of their epitopes for treatment with trypsin, resulted in the identification of one non-neutralizing antibody in each panel that binds to a trypsin-sensitive epitope. Furthermore, each of these antibodies recognized 27 isolates of different provenance, representative of six serotypes. These antibodies are recommended for type-independent antigen detection by ELISA. The epitopes for these antibodies reside at the intertypically conserved N-terminus of capsid protein VP2. The two are specified by the lysines at positions two and three, but differ from each other as indicated by the variable heavy chain sequences of their antibodies.
对针对口蹄疫病毒分离株A22伊拉克/1964、Asia1沙米尔-以色列/1989和SAT1津巴布韦/1989产生的单克隆抗体在中和活性及其表位对胰蛋白酶处理的敏感性方面进行了表征，结果在每个组中鉴定出一种与胰蛋白酶敏感表位结合的非中和抗体。此外，这些抗体中的每一种都识别27种不同来源的分离株，代表六种血清型。推荐这些抗体用于通过ELISA进行与型别无关的抗原检测。这些抗体的表位位于衣壳蛋白VP2典型保守的N端。两者由第2和第3位的赖氨酸指定，但如它们抗体的可变重链序列所示彼此不同。
