Weed D T, Gomez-Fernandez C, Bonfante E, Lee T D, Pacheco J, Carvajal M E, Goodwin W J, Carraway K L
Department of Otolaryngology, University of Miami School of Medicine, Miami, FL 33136, USA.
Otolaryngol Head Neck Surg. 2001 Feb;124(2):127-41. doi: 10.1067/mhn.2001.112575.
This study investigates MUC4 expression in normal squamous epithelia and squamous cell carcinoma (SCC) of the upper aerodigestive tract (UADT), and in salivary gland neoplasms.
MUC4 antigens in tumor and adjacent normal tissue are localized by immunocytochemical studies. Fresh frozen tissues from surgical resection specimens are further analyzed by Western blot.
MUC4 is identified by immunocytochemical staining throughout the normal UADT mucosa, in 34 of 40 primary UADT SCC, and in 11 of 12 metastatic cervical lymph nodes. A trend toward decreased MUC4 staining in moderately and poorly differentiated tumors is noted. Immunoblots show MUC4 in 4 of 5 SCC analyzed. Immunocytochemical staining of MUC4 in 13 major and minor salivary gland neoplasms reveal variable staining of normal and neoplastic tissue. MUC4 is demonstrated in immunoblots of normal parotid tissue and in the single parotid malignancy analyzed, but is not demonstrated in one minor salivary gland malignancy. These findings characterize normal UADT mucosal and salivary MUC4 expression, and MUC4 expression in SCC of the UADT and in salivary gland tumors.
Correlation of MUC4 expression with clinical outcomes may establish MUC4 as a potential molecular prognostic marker for these tumors.
本研究调查MUC4在上呼吸道消化道（UADT）正常鳞状上皮、鳞状细胞癌（SCC）以及涎腺肿瘤中的表达情况。
通过免疫细胞化学研究对肿瘤及相邻正常组织中的MUC4抗原进行定位。手术切除标本的新鲜冷冻组织通过蛋白质印迹法进一步分析。
免疫细胞化学染色显示，MUC4存在于整个正常UADT黏膜、40例原发性UADT SCC中的34例以及12例转移性颈部淋巴结中的11例。观察到在中分化和低分化肿瘤中MUC4染色有降低趋势。蛋白质印迹显示，在分析的5例SCC中有4例存在MUC4。对13例大涎腺和小涎腺肿瘤进行MUC4免疫细胞化学染色，结果显示正常组织和肿瘤组织的染色情况各不相同。在正常腮腺组织和分析的1例腮腺恶性肿瘤的蛋白质印迹中显示有MUC4，但在1例小涎腺恶性肿瘤中未显示。这些发现明确了正常UADT黏膜和涎腺中MUC4的表达情况，以及UADT SCC和涎腺肿瘤中MUC4的表达情况。
MUC4表达与临床结果的相关性可能使MUC4成为这些肿瘤潜在的分子预后标志物。
