Tähtinen M, Strengell M, Collings A, Pitkänen J, Kjerrström A, Hakkarainen K, Peterson P, Kohleisen B, Wahren B, Ranki A, Ustav M, Krohn K
Institute of Medical Technology, Tampere University, 33014, Tampere, Finland.
Vaccine. 2001 Feb 28;19(15-16):2039-47. doi: 10.1016/s0264-410x(00)00420-5.
The immunogenicity of a self-replicating DNA-vector containing HIV-1 nef gene (pBN-Nef) was characterized using various DNA delivery methods. In addition, gene gun immunisation was used for assessing immunogenicity of two other HIV-1 genes (rev and tat) given in the same vector. The pBN-Nef was the most immunogenic raising both humoral and cell-mediated immune responses in mice; these responses lasted for up to six months. The pBN-Nef vector was immunogenic also when given intramuscularly or intradermally. The pBN-Rev construct did not elicit humoral responses but did elicit proliferative as well as CTL-response against the corresponding protein. The pBN-Tat was a poor immunogen in all respects. The antibodies elicited with various DNA delivery methods belonged to different antibody subclasses; however, two main epitopes in Nef were frequently recognized by all of them.
利用多种DNA递送方法对含有HIV-1 nef基因的自我复制DNA载体（pBN-Nef）的免疫原性进行了表征。此外，基因枪免疫用于评估同一载体中另外两个HIV-1基因（rev和tat）的免疫原性。pBN-Nef免疫原性最强，可在小鼠中引发体液免疫和细胞介导的免疫反应；这些反应可持续长达六个月。pBN-Nef载体经肌肉注射或皮内注射时也具有免疫原性。pBN-Rev构建体未引发体液反应，但确实引发了针对相应蛋白质的增殖反应以及CTL反应。pBN-Tat在各方面都是一种弱免疫原。通过各种DNA递送方法引发的抗体属于不同的抗体亚类；然而，Nef中的两个主要表位经常被所有抗体识别。
