Burgeot C, Gilbert F B, Poutrel B
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380, Nouzilly, France.
Vaccine. 2001 Feb 28;19(15-16):2092-9. doi: 10.1016/s0264-410x(00)00402-3.
Immunopotentiation of Staphylococcus aureus type 5 capsular polysaccharide (CP5) by use of liposomes as an alternative to protein-polysaccharide conjugates was investigated. Mice were immunized twice with cationic liposomes containing CP5 alone or CP5 co-entrapped with alpha-toxin or heat-detoxified alpha-toxin. Immunogenicity of these different antigens was compared with CP5-alpha-toxin conjugates. Antibodies against CP5 were elicited in mice immunized with conjugates or liposomes containing co-entrapped CP5 and alpha-toxin. Liposomes containing CP5 alone or co-entrapped CP5 and alpha-toxoid failed to induce antibodies against CP5. All the preparations entailed an antibody response against alpha-toxin and highest antibody and neutralizing activity titers were obtained with liposomes. These results show that liposomes can be used to immunopotentiate CP5, however, a co-incorporated protein able to insert lipids bilayers is probably required.
研究了使用脂质体作为蛋白质 - 多糖缀合物的替代物对金黄色葡萄球菌5型荚膜多糖（CP5）进行免疫增强作用。用仅含有CP5或与α - 毒素或热解毒α - 毒素共包封的CP5的阳离子脂质体对小鼠进行两次免疫。将这些不同抗原的免疫原性与CP5 - α - 毒素缀合物进行比较。在用缀合物或含有共包封的CP5和α - 毒素的脂质体免疫的小鼠中引发了针对CP5的抗体。仅含有CP5或共包封CP5和类毒素的脂质体未能诱导针对CP5的抗体。所有制剂都引发了针对α - 毒素的抗体反应，并且脂质体获得了最高的抗体和中和活性滴度。这些结果表明脂质体可用于免疫增强CP5，然而，可能需要一种能够插入脂质双层的共掺入蛋白质。
