Parveen S, Hodge N C, Stall R E, Farrah S R, Tamplin M L
Departments of Microbiology and Cell Science, P.O. Box. 110700, University of Florida, Gainesville, FL 32611-0700, USA.
Water Res. 2001 Feb;35(2):379-86. doi: 10.1016/s0043-1354(00)00269-4.
Estuarine waters receive fecal pollution from a variety of sources, including humans and wildlife. Escherichia coli is one of several fecal coliform bacteria that inhabit the intestines of many warm-blooded animals that sometimes contaminate water. Its presence does not specifically implicate human fecal input, therefore it is necessary to differentiate contamination sources to accurately assess health risks. E. coli were isolated from human sources (HS) and nonhuman sources (NHS) in the Apalachicola National Estuarine Research Reserve and analyzed for fatty acid methyl ester (FAME), O-serogroup, and pulsed-field gel electrophoresis (PFGE) profiles. For FAME and PFGE analyses, there was no relationship between profile and isolate source. Human source PFGE profiles were less diverse than NHS isolates, and conversely for FAME. In contrast, O-serogrouping showed less diversity for HS vs. NHS isolates, and the predominant HS O-serogroups differed significantly (P < 0.01) from those of NHS isolates.
河口水域受到来自包括人类和野生动物在内的多种来源的粪便污染。大肠杆菌是几种粪便大肠菌群细菌之一，存在于许多温血动物的肠道中，有时会污染水源。它的存在并不特别意味着人类粪便的输入，因此有必要区分污染源以准确评估健康风险。在阿巴拉契科拉国家河口研究保护区从人类来源（HS）和非人类来源（NHS）分离出大肠杆菌，并分析其脂肪酸甲酯（FAME）、O-血清群和脉冲场凝胶电泳（PFGE）图谱。对于FAME和PFGE分析，图谱与分离株来源之间没有关系。人类来源的PFGE图谱比NHS分离株的多样性更低，而FAME则相反。相比之下，O-血清群分析显示HS分离株与NHS分离株相比多样性更低，并且主要的HS O-血清群与NHS分离株的有显著差异（P < 0.01）。
