通过聚合酶链反应对猪组织中的猪巨细胞病毒进行定量分析。
Quantitation of porcine cytomegalovirus in pig tissues by PCR.
作者信息
Fryer J F, Griffiths P D, Fishman J A, Emery V C, Clark D A
机构信息
Department of Virology, Royal Free and University College Medical School of UCL, Rowland Hill Street, London NW3 2PF, United Kingdom.
出版信息
J Clin Microbiol. 2001 Mar;39(3):1155-6. doi: 10.1128/JCM.39.3.1155-1156.2001.
Abstract
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/microg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.
摘要
开发了一种用于定量猪巨细胞病毒（PCMV）的定量竞争PCR方法。在多种猪器官（包括潜在的异种移植供体）中检测到该病毒，病毒载量范围为每微克DNA小于10至97个基因组拷贝。该检测方法在研究PCMV的激活和复制以及在同种异体和异种移植的猪模型中将具有重要用途。
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1
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J Virol Methods. 2000 Jun;87(1-2):177-81. doi: 10.1016/s0166-0934(00)00164-6.
2
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J Clin Microbiol. 1999 Nov;37(11):3767-8. doi: 10.1128/JCM.37.11.3767-3768.1999.
3
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4
Infection and xenotransplantation. Developing strategies to minimize risk.感染与异种移植。制定将风险降至最低的策略。
Ann N Y Acad Sci. 1998 Dec 30;862:52-66. doi: 10.1111/j.1749-6632.1998.tb09117.x.
5
Infection in organ-transplant recipients.器官移植受者中的感染
N Engl J Med. 1998 Jun 11;338(24):1741-51. doi: 10.1056/NEJM199806113382407.
6
Xenogeneic transplantation.异种移植
Annu Rev Immunol. 1998;16:433-70. doi: 10.1146/annurev.immunol.16.1.433.
7
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8
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Kidney Int Suppl. 1997 Mar;58:S41-5.
9
Infection of human cells by an endogenous retrovirus of pigs.猪内源性逆转录病毒对人类细胞的感染。
Nat Med. 1997 Mar;3(3):282-6. doi: 10.1038/nm0397-282.
10
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J Gen Virol. 1996 Sep;77 ( Pt 9):2271-5. doi: 10.1099/0022-1317-77-9-2271.
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