Fukushima E, Arata Y, Endo T, Sonnewald U, Sato F
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502 Japan.
Plant Cell Physiol. 2001 Feb;42(2):245-9. doi: 10.1093/pcp/pce027.
We investigated the salt tolerance of transgenic tobacco, in which yeast invertase is expressed in the apoplastic (Apo-Inv) spaces. Whereas photosynthetic activities in wild-type tobacco in light were inhibited under salt stress, transgenic Apo-Inv tobacco maintained constant photosynthetic activities. The physical appearance of plants under salt stress also indicates that yeast invertase expression in the apoplastic space is beneficial for inducing salt tolerance. Apo-Inv tobacco had a much higher osmotic pressure increase in the cell sap than did wild-type tobacco under this type of stress. The physiological importance of sucrose metabolism under salt stress is discussed.
我们研究了在质外体（Apo-Inv）空间中表达酵母转化酶的转基因烟草的耐盐性。在盐胁迫下，野生型烟草在光照下的光合活性受到抑制，而转基因Apo-Inv烟草的光合活性保持恒定。盐胁迫下植株的外观也表明，质外体空间中酵母转化酶的表达有利于诱导耐盐性。在这种胁迫下，Apo-Inv烟草细胞液中的渗透压升高幅度比野生型烟草高得多。本文还讨论了盐胁迫下蔗糖代谢的生理重要性。
