Lefevre S, Debat H, Thomas D, Friboulet A, Avalle B
Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, P.O. Box 20529, 60205 Cedex, Compiegne, France.
FEBS Lett. 2001 Jan 26;489(1):25-8. doi: 10.1016/s0014-5793(01)02075-0.
The catalytic mechanism of an anti-idiotypic antibody, 9G4H9, displaying a beta-lactamase activity was investigated. Kinetics experiments suggest that some penicillinic derivatives behave both as substrates and inactivators. Biochemical and immunological experiments strongly indicate that ampicillin may be regarded as a suicide substrate for hydrolysis by 9G4H9. The anti-idiotypic network appears as a way to create enzyme mimics with modified catalytic activities.
