Simon H M, Dodsworth J A, Goodman R M
Department of Plant Pathology, University of Wisconsin-Madison, 53706, USA.
Environ Microbiol. 2000 Oct;2(5):495-505. doi: 10.1046/j.1462-2920.2000.00131.x.
Microorganisms that colonize plant roots are recruited from, and in turn contribute substantially to, the vast and virtually uncharacterized phylogenetic diversity of soil microbiota. The diverse, but poorly understood, microorganisms that colonize plant roots mediate mineral transformations and nutrient cycles that are central to biosphere functioning. Here, we report the results of epifluorescence microscopy and culture-independent recovery of small subunit (SSU) ribosomal RNA (rRNA) gene sequences showing that members of a previously reported clade of soil Crenarchaeota colonize both young and senescent plant roots at an unexpectedly high frequency, and are particularly abundant on the latter. Our results indicate that non-thermophilic members of the Archaea inhabit an important terrestrial niche on earth and direct attention to the need for studies that will determine their possible roles in mediating root biology.
定殖于植物根系的微生物源自土壤微生物群中庞大且几乎未被描述的系统发育多样性，反过来又对其做出了重大贡献。定殖于植物根系的微生物种类多样，但人们对它们了解甚少，它们介导着对生物圈功能至关重要的矿物质转化和养分循环。在这里，我们报告了落射荧光显微镜检查的结果以及从小亚基（SSU）核糖体RNA（rRNA）基因序列中进行的非培养回收结果，这些结果表明，先前报道的土壤泉古菌一个进化枝的成员以出乎意料的高频率定殖于幼嫩和衰老的植物根系，并且在后者上尤为丰富。我们的结果表明，古菌的非嗜热成员在地球上占据着重要的陆地生态位，并促使人们关注开展相关研究的必要性，这些研究将确定它们在介导根系生物学过程中可能发挥的作用。
