[人类中期染色体的原位引物标记]
[Primed in situ labelling of human metaphase chromosomes].
作者信息
Guo Y Q, Cheng H H, Huang X, Zhou R J
机构信息
College of Life Sciences, Wuhan University, Wuhan 430072, China.
出版信息
Yi Chuan Xue Bao. 2001;28(2):103-6.
Primed in situ labelling (PRINS) contributes to the elucidation of the organization of eukaryotic chromosomes and provides an alternative way to localize gene on chromosomes. Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. The particular band pattern of dark and light was observed by using random primer PRINS. SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes.
原位引物标记法（PRINS）有助于阐明真核生物染色体的组织结构，并为在染色体上定位基因提供了一种替代方法。在此，我们展示了随机引物和SOX简并引物PRINS应用于人类中期染色体的结果。使用随机引物PRINS观察到了明暗相间的特定条带模式。SOX简并引物PRINS揭示了更多的SOX基因座，并进一步证明SOX基因在人类染色体上并非成簇分布。
Zotero 插件