鸡的八个SOX家族基因的染色体定位

Chromosome assignment of eight SOX family genes in chicken.

作者信息

Kuroiwa A, Uchikawa M, Kamachi Y, Kondoh H, Nishida-Umehara C, Masabanda J, Griffin D K, Matsuda Y

机构信息

Laboratory of Animal Cytogenetics, Center for Advanced Science and Technology, Hokkaido University, Sapporo, Japan.

出版信息

Cytogenet Genome Res. 2002;98(2-3):189-93. doi: 10.1159/000069803.

DOI:10.1159/000069803

PMID:12698002

Abstract

Chromosome locations of the eight SOX family genes, SOX1, SOX2, SOX3, SOX5, SOX9, SOX10, SOX14 and SOX21, were determined in the chicken by fluorescence in situ hybridization. The SOX1 and SOX21 genes were localized to chicken chromosome 1q3.1-->q3.2, SOX5 to chromosome 1p1.6-->p1.4, SOX10 to chromosome 1p1.6, and SOX3 to chromosome 4p1.2-->p1.1. The SOX2 and SOX14 genes were shown to be linked to chromosome 9 using two-colored FISH and chromosome painting, and the SOX9 gene was assigned to a pair of microchromosomes. These results suggest that these SOX genes form at least three clusters on chicken chromosomes. The seven SOX genes, SOX1, SOX2, SOX3, SOX5, SOX10, SOX14 and SOX21 were localized to chromosome segments with homologies to human chromosomes, indicating that the chromosome locations of SOX family genes are highly conserved between chicken and human.

摘要

通过荧光原位杂交技术确定了鸡体内8个SOX家族基因，即SOX1、SOX2、SOX3、SOX5、SOX9、SOX10、SOX14和SOX21的染色体定位。SOX1和SOX21基因定位于鸡的1号染色体1q3.1→q3.2区域，SOX5定位于1号染色体1p1.6→p1.4区域，SOX10定位于1号染色体1p1.6区域，SOX3定位于4号染色体4p1.2→p1.1区域。利用双色荧光原位杂交和染色体涂染技术显示，SOX2和SOX14基因与9号染色体相连，SOX9基因定位于一对微小染色体上。这些结果表明，这些SOX基因在鸡的染色体上至少形成了三个基因簇。SOX1、SOX2、SOX3、SOX5、SOX10、SOX14和SOX21这7个SOX基因定位于与人类染色体具有同源性的染色体片段上，这表明SOX家族基因在鸡和人类之间的染色体定位具有高度保守性。