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[节节麦异源多倍体物种基因组进化的随机扩增多态性DNA分析]

[RAPD analysis of the genome evolution in allopolyploid species in Aegilops].

作者信息

Cai C L, Wang J B, Jing R C, Zhu Y G

机构信息

College of Life Sciences, Wuhan University, Wuhan 430072, China.

出版信息

Yi Chuan Xue Bao. 2001;28(2):158-65.

Abstract

RAPD analysis was performed by applying a set of 24 arbitrary primers to all the allopolyploids and their diploid progenitors in Aegilops L. The analysis based on 313 RAPD fragments revealed that the allopolyploids carrying D genome grouped with Ae. squarrosa (DD) in one cluster. The allopolyploids carrying U genome grouped with Ae. umbellulata (UU) in another cluster except Ae. juvenalis (DDMMUU), which grouped with Ae. squarrosa (DD). The allopolyploids did not cluster with the other diploids. The results indicated that the allopolyploids shared close relationship with Ae. squarrosa (DD) and Ae. umbellulata (UU), respectively. It proved that the D and U genomes altered relatively little from the diploid progenitors after the allopolyploids came into being, but the other genomes changed greatly.

摘要

利用一组24个任意引物对山羊草属的所有异源多倍体及其二倍体祖先进行了随机扩增多态性DNA(RAPD)分析。基于313个RAPD片段的分析表明,携带D基因组的异源多倍体与节节麦(DD)聚为一个簇。携带U基因组的异源多倍体除了青年山羊草(DDMMUU)与节节麦(DD)聚在一起外,其余与伞穗山羊草(UU)聚为另一个簇。异源多倍体没有与其他二倍体聚在一起。结果表明,异源多倍体分别与节节麦(DD)和伞穗山羊草(UU)关系密切。这证明异源多倍体形成后,D和U基因组与其二倍体祖先相比变化相对较小,而其他基因组变化很大。

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