He C Y, Zhang Z Y, Wang Y J, Zheng X W, Yu D Y, Chen S Y, Gai J Y
Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan Xue Bao. 2001;28(2):171-81.
The prerequisite of genomic research such as genetic mapping is construction of a suitable segregating population and its reasonable evaluation before use. A recombinant inbred line (RILs), NJRIKY, was constructed through a cross between Kefeng 1 and Nannong 1138-2. The present study was focused on evaluation of genetic structure of the RILs through the simple sequence repeats (SSRs) analysis. A total number of 138 pairs of SSRs primers covering the whole soybean genome were randomly selected for polymorphic analysis between two parents. 86 pairs of which were polymorphic and polymorphic rate is up to 62.32%. 90 polymorphic loci were found. Further analysis with polymorphic SSRs loci on the sampled lines indicated that not only segregation of most SSRs loci conformed to the ratio of 1:1, but also almost each sampled line tended to homozyge. The distribution of genotypic composition was normal. The population is ideal for genetic mapping and other genomic research.
诸如基因定位等基因组研究的前提是构建合适的分离群体并在使用前对其进行合理评估。通过科丰1号和南农1138 - 2杂交构建了一个重组自交系群体（NJRIKY）。本研究聚焦于通过简单序列重复（SSR）分析评估该重组自交系群体的遗传结构。随机选择了覆盖整个大豆基因组的138对SSR引物对两个亲本进行多态性分析。其中86对具有多态性，多态率高达62.32%。共发现90个多态性位点。对抽样品系上的多态性SSR位点进行进一步分析表明，不仅大多数SSR位点的分离符合1:1的比例，而且几乎每个抽样品系都趋于纯合。基因型组成的分布是正常的。该群体对于基因定位和其他基因组研究来说是理想的。
