Wu X L, He C Y, Wang Y J, Zhang Z Y, Dongfang Y, Zhang J S, Chen S Y, Gai J Y
Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan Xue Bao. 2001 Nov;28(11):1051-61.
A high-density genetic map was constructed with a RILs population (NJRIKY) from a cross of two cultivated soybean Kefeng 1 and Nannong 1138-2, using RFLP, SSR, RAPD and AFLP markers. The map consists of 24 linkage groups, which include 792 genetic markers, and covers 2,320.7 cM with an average genetic distance of 2.9 cM. The higher polymorphsims between two parents were identified with SSR markers. Due to its simplicity and stability, they could be used as anchored marker to integrate different maps constructed with different genetic markers. AFLP marker system is efficient for map construction while it easily form clusters to cause big gaps in the map. A total of 21.7% of markers was found with distorted segregation in the RILs population. The high-density molecular linkage map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits.
利用RFLP、SSR、RAPD和AFLP标记，构建了由两个栽培大豆品种科丰1号和南农1138-2杂交产生的重组自交系群体（NJRIKY）的高密度遗传图谱。该图谱由24个连锁群组成，包含792个遗传标记，覆盖2320.7厘摩，平均遗传距离为2.9厘摩。通过SSR标记鉴定出两个亲本之间具有较高的多态性。由于其简单性和稳定性，SSR标记可作为锚定标记，用于整合用不同遗传标记构建的不同图谱。AFLP标记系统在图谱构建方面效率较高，但容易形成聚类，导致图谱中出现较大间隙。在重组自交系群体中，共发现21.7%的标记存在偏分离现象。高密度分子连锁图谱是基因定位、比较基因组学以及重要农艺性状QTL定位的基础。
