Timtcheva I, Maximova V, Deligeorgiev T, Gadjev N, Drexhage K H, Petkova I
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.
J Photochem Photobiol B. 2000 Nov;58(2-3):130-5. doi: 10.1016/s1011-1344(00)00116-0.
The fluorescence properties of newly synthesized homodimeric monomethine cyanine dyes in the presence of biopolymers are investigated. They do not fluoresce in TE buffer and bidistilled water but become strongly fluorescent (Q(F)=0.3-0.9) in the region 530-650 nm when bound to dsDNA and ssDNA. The detection limit of dsDNA is about 1.7 ng/ml. Some of dyes studied are able to distinguish between dsDNA and ssDNA, RNA, BSA in solution and gel electrophoresis. The influence of different factors (temperature, pH and viscosity of the medium, presence of histone) on the formation of the dye-biopolymer complexes is investigated. The results of steady-state and dynamic fluorescence measurements concerning the different types of binding between dyes and biopolymers show that the new dyes are applicable in molecular biology as highly sensitive fluorescence labels.
研究了新合成的同二聚体单甲川花菁染料在生物聚合物存在下的荧光特性。它们在TE缓冲液和双蒸水中不发荧光，但与双链DNA和单链DNA结合时，在530 - 650 nm区域会发出强荧光（量子产率Q(F)=0.3 - 0.9）。双链DNA的检测限约为1.7 ng/ml。所研究的一些染料能够在溶液和凝胶电泳中区分双链DNA和单链DNA、RNA、牛血清白蛋白。研究了不同因素（温度、介质的pH值和粘度、组蛋白的存在）对染料 - 生物聚合物复合物形成的影响。关于染料与生物聚合物之间不同类型结合的稳态和动态荧光测量结果表明，这些新染料作为高灵敏度荧光标记物可应用于分子生物学领域。
