Dey P, Luthra U K, George S S, Haji B I, George J
Cytology Unit, Department of Pathology, Mubarak Al Kabeer Hospital, and Faculty of Medicine, Kuwait University, Kuwait.
Anal Quant Cytol Histol. 2001 Feb;23(1):27-30.
To study the relationship of cell death and proliferation to histologic grade and p53 expression in invasive carcinoma of the breast.
A total of 31 cases of infiltrating duct carcinoma of the breast were randomly selected. The terminal deoxynucleotidyl-transferase-mediated dUTP nick end labelling (TUNEL) reaction and p53 immunostaining were performed on representative paraffin-embedded tissue sections. Mitotic and apoptotic indices (MI and AI) were also measured on hematoxylin-eosin-stained sections. Histologic grade of infiltrating duct carcinoma was performed with the help of the Nottingham modification of the Bloom-Richardson system. Tumor grade and p53 overexpression were correlated with MI, AI and AI detected by TUNEL.
There were a total of 31 infiltrating duct carcinomas of the breast, of which 13 cases were grade 1 and nine cases each were grade 2 and 3. Cells with positive TUNEL showed a strong brown nuclear positivity. TUNEL showed positivity from the periphery of the nuclear margin to the central portion. AI detected by TUNEL did not correlate with tumor grade (ANOVA, P > .05). MI was significant only in grade 1 versus grade 3 and 2 versus grade 3 carcinomas (ANOVA, P < .01). The morphologic apoptotic index was significant only in grade 1 versus grade 3 carcinomas. Nine cases showed p53 overexpression, and the rest of the cases were negative for p53 immunostaining. MI, AI and TUNEL were not significantly different in p53-negative and -positive groups. Pearson's correlation coefficient showed that AI and MI were significantly related, but there was no significant relation between AI detected by TUNEL and MI.
MI is still more useful than AI or AI detected by TUNEL in differentiating various grades of carcinoma of the breast.
研究乳腺浸润性癌中细胞死亡和增殖与组织学分级及p53表达的关系。
随机选取31例乳腺浸润性导管癌。对代表性石蜡包埋组织切片进行末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）反应和p53免疫染色。在苏木精-伊红染色切片上也测量有丝分裂指数和凋亡指数（MI和AI）。浸润性导管癌的组织学分级借助Bloom-Richardson系统的诺丁汉改良法进行。肿瘤分级和p53过表达与MI、AI以及TUNEL检测的AI相关。
共有31例乳腺浸润性导管癌，其中13例为1级，2级和3级各9例。TUNEL阳性细胞显示强棕色核阳性。TUNEL显示从核边缘外周至中央部分呈阳性。TUNEL检测的AI与肿瘤分级无关（方差分析，P>.05）。MI仅在1级与3级以及2级与3级癌之间有显著差异（方差分析，P<.01）。形态学凋亡指数仅在1级与3级癌之间有显著差异。9例显示p53过表达，其余病例p53免疫染色为阴性。p53阴性和阳性组的MI、AI和TUNEL无显著差异。Pearson相关系数显示AI和MI显著相关，但TUNEL检测的AI与MI之间无显著关系。
在区分不同分级的乳腺癌方面，MI比AI或TUNEL检测的AI更有用。
