Bailey S R, Cunningham F M
Department of Veterinary Basic Sciences, The Royal Veterinary College, Hatfield, Hertfordshire, UK.
Inflamm Res. 2001 Jan;50(1):32-8. doi: 10.1007/s000110050721.
To compare adherence of stimulated and unstimulated eosinophils from allergic and normal ponies to cultured equine vascular endothelial cells (equine digital vein endothelial cells; EDVEC) and examine the effect of eosinophil-derived factor(s) on cell adherence.
Eosinophil adherence to unstimulated EDVEC or EDVEC pretreated with IL-1beta or supernatants from stimulated eosinophils was measured. Supernatants were also assayed for TNFalpha and IL-1beta-like bioactivity.
Adherence of unstimulated and rhIL-5 (10 ng/ml)-stimulated eosinophils from allergic ponies to rhIL-1beta-treated EDVEC was significantly greater than that of cells from normal ponies. Pretreatment of EDVEC with supernatants from stimulated eosinophils from both groups of ponies significantly increased adherence of autologous cells and IL-1beta- and TNFalpha-like bioactivities were detected in the supernatants.
Mediator-induced activation of equine eosinophils may lead to further eosinophil recruitment by releasing cytokines that up-regulate endothelial cell adhesion molecule expression. Increased adherence of blood eosinophils from allergic ponies to stimulated endothelium could be explained by in vivo priming.
比较变应性和正常小马的受刺激与未受刺激嗜酸性粒细胞对培养的马血管内皮细胞（马趾静脉内皮细胞；EDVEC）的黏附情况，并研究嗜酸性粒细胞衍生因子对细胞黏附的影响。
检测嗜酸性粒细胞对未受刺激的EDVEC或用IL-1β预处理的EDVEC或受刺激嗜酸性粒细胞的上清液的黏附情况。还对上清液检测了TNFα和IL-1β样生物活性。
变应性小马未受刺激的和经rhIL-5（10 ng/ml）刺激的嗜酸性粒细胞对经rhIL-1β处理的EDVEC的黏附显著高于正常小马的细胞。用两组小马受刺激嗜酸性粒细胞的上清液预处理EDVEC显著增加了自体细胞的黏附，且在上清液中检测到IL-1β和TNFα样生物活性。
介质诱导的马嗜酸性粒细胞活化可能通过释放上调内皮细胞黏附分子表达的细胞因子导致进一步的嗜酸性粒细胞募集。变应性小马血液嗜酸性粒细胞对受刺激内皮的黏附增加可通过体内致敏来解释。
