[bcl-2基因家族在紫杉醇介导的BJAB B细胞淋巴瘤细胞系凋亡中的作用]
[The role of bcl-2 gene family in taxol-mediated apoptosis in BJAB B cell lymphoma cell line].
作者信息
Zhou X, Xu L, He K
机构信息
Laboratory of Molecular Pathology, Cancer Hospital, Shanghai Medical University, Shanghai 200032, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2000 Nov;22(6):453-5.
OBJECTIVE
To study the apoptosis-inducing effect of taxol on BJAB B cell lymphoma cell line and the role of bcl-2 gene family.
METHODS
BJAB cells were treated with taxol and examined for morphologic changes under light and electron microscopes. Flow cytometry assays and DNA gel electrophoresis were used to demonstrate changes in nuclear DNA contents and DNA fragmentations. Changes in bcl-2 gene family at mRNA and protein levels were studied by immunohistochemistry and semi-quantitative RT-PCR technique.
RESULTS
Taxol inhibited growth of BJAB cells. The cells initially showed G2/M arrest, followed by apoptosis, in a time and dose dependent manner. The expression of bcl-2 at mRNA and protion level was decreased while that of bcl-xs transcription was increased after taxol treatment.
CONCLUSION
Taxol induces apoptosis of BJAB cells. Bcl-2 and bcl-xs are involved in the regulation of taxol-induced apoptosis.
目的
研究紫杉醇对BJAB B细胞淋巴瘤细胞系的凋亡诱导作用及bcl-2基因家族的作用。
方法
用紫杉醇处理BJAB细胞，在光镜和电镜下观察其形态变化。采用流式细胞术检测和DNA凝胶电泳来证明核DNA含量和DNA片段化的变化。通过免疫组织化学和半定量RT-PCR技术研究bcl-2基因家族在mRNA和蛋白质水平的变化。
结果
紫杉醇抑制BJAB细胞的生长。细胞最初表现为G2/M期阻滞，随后以时间和剂量依赖的方式发生凋亡。紫杉醇处理后，bcl-2在mRNA和蛋白质水平的表达降低而bcl-xs转录增加。
结论
紫杉醇诱导BJAB细胞凋亡。Bcl-2和bcl-xs参与紫杉醇诱导凋亡的调控。
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