荧光原位杂交技术（FISH）在肺癌痰细胞学诊断中的应用
[Application of fluorescence in situ hybridization (FISH) in sputum cytologic diagnosis of lung cancer].
作者信息
Jia D, Zhang Z, Liu S
机构信息
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2000 Nov;22(6):477-9.
OBJECTIVE
To study the numerical chromosomal abnormalities of cells in sputum from patients with lung cancer by dual-fluorescence in situ hybridization (FISH).
METHODS
Thirty sputum samples from lung cancer patients were examined by FISH with centromere DNA probes of chromosome 7, 11, 17 and X.
RESULTS
In 23 positive sputum samples studied, the frequency of hyperdiploid of chromosome 7, 17, X and 11 was 65.2% (15/23), 60.9% (14/23), 52.2% (12/23) and 39.1% (9/23), respectively. Hyperdiploidy of chromosome 7 was found in 4 of 7 sputum samples which were cytologicaly suspicious of cancer cells.
CONCLUSION
FISH can detect aneuploid malignant cells in sputum and be used as a complementary technique to cytologic diagnosis of lung cancer.
目的
通过双荧光原位杂交（FISH）研究肺癌患者痰液中细胞的染色体数目异常情况。
方法
采用染色体7、11、17和X的着丝粒DNA探针，对30份肺癌患者的痰液样本进行FISH检测。
结果
在23份阳性痰液样本中，染色体7、17、X和11超二倍体的频率分别为65.2%（15/23）、60.9%（14/23）、52.2%（12/23）和39.1%（9/23）。在7份细胞学检查怀疑有癌细胞的痰液样本中，有4份检测到染色体7超二倍体。
结论
FISH可检测痰液中的非整倍体恶性细胞，可作为肺癌细胞学诊断的补充技术。
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