[人类肺癌染色体非整倍体的研究]
[The study on the chromosome aneuploidy in human lung cancer].
作者信息
Gao Shu-geng, Dong Xiang-yang, Wang Liang-jun, He Jie
机构信息
Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2007 Jun 26;87(24):1701-3.
OBJECTIVE
To study the feasibility of interphase fluorescence in situ hybridization (FISH) in detection of chromosomal aneuploidy in touch preparations of lung carcinoma and its clinic significance.
METHODS
We examined 46 touch preparations of lung carcinomas by FISH with DNA specific for chromosomes 7, 8, 9 and 12 to detect chromosomal aneuploidy.
RESULTS
Aneuploidy, mainly featured in gains of chromosomes 7, 8 and 12 and loss of chromosome 9 were observed in all of the touch preparations of the 46 cases with lung cancer. The rates of chromosomal aneuploidy detected by FISH were 67.4% (31/46), 60.9% (28/46), 28.3% (13/46) and 56.5% (26/31) respectively.
CONCLUSION
Interphase FISH is feasible in detecting aneuploidy in touch preparations of lung cancer. These alterations may be applied to early diagnosis and predicting prognosis of lung cancer.
目的
探讨间期荧光原位杂交技术（FISH）检测肺癌印片染色体非整倍体的可行性及其临床意义。
方法
采用针对7、8、9和12号染色体的DNA探针，应用FISH技术检测46例肺癌印片的染色体非整倍体。
结果
46例肺癌印片均存在非整倍体，主要表现为7、8和12号染色体增多及9号染色体减少。FISH检测染色体非整倍体的阳性率分别为67.4%（31/46）、60.9%（28/46）、28.3%（13/46）和56.5%（26/31）。
结论
间期FISH技术检测肺癌印片染色体非整倍体是可行的，这些改变可能有助于肺癌的早期诊断和预后判断。
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