Hewitt J A
J Gen Virol. 1975 Jan;26(1):87-94. doi: 10.1099/0022-1317-26-1-87.
Satellite or defective bacteriophage particles can appear in extensively recycled stocks of coliphage M13. These particles, herein known as miniphage, replicate using the wild type bacteriophage as a helper. Their physical properties (u.v. spectra, sedimentation of DNA and bacterophage, electrophoretic moblitiy) are described and a method for the isolation of specific satellite bacteriophage is presented.
在大量循环使用的大肠杆菌噬菌体M13菌液中可能会出现卫星噬菌体或缺陷噬菌体颗粒。这些颗粒,在此被称为微小噬菌体,利用野生型噬菌体作为辅助进行复制。描述了它们的物理特性(紫外光谱、DNA和噬菌体的沉降、电泳迁移率),并提出了一种分离特定卫星噬菌体的方法。