Lin L Z, Wu C L, Lu Z, Huang Y R, Zhuang J G, Zhou Z N
NMR Laboratory, Analytic Center, East China Normal University, Shanghai 200062.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2000 Nov;16(4):324-6.
To assess the myocardial effects of Salvia Miltiorrhiza (SM) injection against hypoxia-reoxygenation injury, 31P NMR was used to trace the time courses of high energy phosphates (HEP) content and intracellular pH (pHi) of the isolated perfused rat hearts under hypoxia (30 min) and subsequent reoxygenation (40 min).
It was discovered that SM significantly preven-ted the decrease in the myocardial HEP content during hypoxia, enhanced the recovery of myocardial phosphocreatine (PCr), adenosine triphosphate (ATP) and the PCr/Pi (inorganic phosphate) ratios during reoxygenation, and lightened the decrease of the myocardial pHi value caused by hypoxia.
SM improves the myocardial energy metabolism level during prolonged hypoxia and subsequent reoxygenation and protects myocardium against hypoxia/reoxygenation injury. SM significantly attenuates acidosis during hypoxia and prevents the appearance of very acidic areas of the myocardium after reoxygenation.
为评估丹参注射液对缺氧-复氧损伤的心肌效应，采用31P核磁共振技术追踪离体灌注大鼠心脏在缺氧（30分钟）及随后复氧（40分钟）过程中高能磷酸盐（HEP）含量和细胞内pH（pHi）的时间变化过程。
发现丹参显著预防了缺氧期间心肌HEP含量的降低，增强了复氧期间心肌磷酸肌酸（PCr）、三磷酸腺苷（ATP）的恢复以及PCr/无机磷酸盐（Pi）比值，并减轻了缺氧引起的心肌pHi值下降。
丹参可改善长时间缺氧及随后复氧期间的心肌能量代谢水平，保护心肌免受缺氧/复氧损伤。丹参显著减轻缺氧期间的酸中毒，并防止复氧后心肌出现极酸性区域。
