Lee T J, Westfall D P, Fleming W W
J Pharmacol Exp Ther. 1975 Jan;192(1):136-48.
Chronic postganglionic denervation of the rate vas deferens produces an increase in the sensitivity of the in vitro smooth muscle to norepinephrine, methoxamine, acetylcholine, potassium and electrical stimulation. The maximal contractile response to norepinephrine and acetylcholine is increased but the maximal response to potassium or electrical stimulation is not affected. In addition, the vas deferens becomes spontaneously active after denervation. The spontaneous contractions are not prevented by tetrodotoxin, phentolamine or atropine. The appearance and magnitude of the spontaneous contractions are temporally correlated with the development of postjunctional supersensitivity. At a given time after denervation there is a significant correlation between the magnitude of the spontaneous contractions and the sensitivity to norepinephrine, i.e., the sensitivity to norepinephrine being greatest in those tissues with the largest spontaneous contractions. There is an inverse relationship between the magnitude of the spontaneous contractions and the endogenous norepinephrine concentration. Pretreatment of rats with reserpine (1. 0 mg/kg/day for 5-7 days) results in supersensitivity of the vas deferens ,o norepinephrine and acetylcholine. Those tissues obtained from reserpine-pretreated rats which are supersensitive are also spontaneously active. It is concluded that the -ppearance of spontaneous contractions in the rat vas deferens is an index of postjunctional supersensitivity.
输精管节后慢性去神经支配会使体外平滑肌对去甲肾上腺素、甲氧明、乙酰胆碱、钾和电刺激的敏感性增加。对去甲肾上腺素和乙酰胆碱的最大收缩反应增强，但对钾或电刺激的最大反应不受影响。此外，输精管去神经支配后会出现自发活动。河豚毒素、酚妥拉明或阿托品不能阻止自发收缩。自发收缩的出现和幅度与节后超敏反应的发展在时间上相关。去神经支配后的特定时间，自发收缩的幅度与对去甲肾上腺素的敏感性之间存在显著相关性，即自发收缩最大的那些组织对去甲肾上腺素的敏感性最高。自发收缩的幅度与内源性去甲肾上腺素浓度呈负相关。用利血平（1.0毫克/千克/天，持续5 - 7天）预处理大鼠会导致输精管对去甲肾上腺素和乙酰胆碱超敏。从利血平预处理大鼠获得的那些超敏组织也有自发活动。结论是大鼠输精管中自发收缩的出现是节后超敏反应的一个指标。
