Westfall D P, Lee T J, Stitzel R E
Fed Proc. 1975 Sep;34(10):1985-9.
The effect of postganglionic denervation on the incidence of nexal contacts in the smooth muscle of the rat vas deferens was investigated. The chronically denervated tissue exhibited twice as many nexuses as control. This increase in the incidence of cell contacts may contribute to the supersensitivity and/or the increase in maximum response of the denervated vas deferens. The effects of denervation, decentralization, and pretreatment with reserpine on the concentration of adenosine triphosphate (ATP) in vasa deferentia of rats and guinea pigs were also investigated. One day after denervation there was a substantial decrease in the endogenous norepinephrine and ATP concentrations. The norepinephrine concentration remained low (less than 10% of control) throughout subsequent days (up to 14 days) whereas the ATP concentration, after the first postoperative day, rose significantly. The rise in ATP concentration was temporally correlated with the development of postjunctional supersensitivity. Decentralization and pretreatment with reserpine both resulted in a significant increase in ATP concentration which preceded by 2 to 3 days a significant increase in sensitivity of the vas deferens. It appears that a change in the tissue concentration of ATP may be one of the initial events that occurs following interruption of the neural contact to the smooth muscle of the vas deferens.
研究了节后去神经支配对大鼠输精管平滑肌中连接复合体发生率的影响。长期去神经支配的组织中连接复合体的数量是对照组的两倍。细胞接触发生率的增加可能导致去神经支配的输精管超敏反应和/或最大反应增加。还研究了去神经支配、中枢去传入和利血平预处理对大鼠和豚鼠输精管中三磷酸腺苷（ATP）浓度的影响。去神经支配一天后，内源性去甲肾上腺素和ATP浓度大幅下降。在随后的几天（长达14天）中，去甲肾上腺素浓度一直很低（低于对照组的10%），而术后第一天后，ATP浓度显著升高。ATP浓度的升高与节后超敏反应的发展在时间上相关。中枢去传入和利血平预处理均导致ATP浓度显著升高，这比输精管敏感性显著升高提前2至3天。看来，ATP组织浓度的变化可能是输精管平滑肌神经联系中断后最初发生的事件之一。
