Truter E J, Santos A S, Els W J
School of Life Sciences, Cape Technikon, Cape Town, South Africa.
Cell Biol Int. 2001;25(1):51-9. doi: 10.1006/cbir.2000.0677.
The 3-(4,5 dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay is used successfully to estimate the number of viable cells in drug screening trials. We used the MTT assay to assess the viability of a rodent ovarian carcinoma cell line (DMBA-OC-1R) after exposure to combinations of cisplatin and 5-fluorouracil as free drug and in encapsulated (conjugated and unconjugated) forms. After 48 h of exposure to free drugs, a significant trend towards cell cytotoxicity could be observed and this was well established by 120 h. Cells treated with drug-containing immuno-microspheres showed a similar initial decrease in cell viability after 96 h, and this was maintained for 128 h. These results suggest that immuno-microspheres loaded with chemotherapeutic drugs have the potential to be successfully used in the treatment of ovarian cancer.
3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法已成功用于药物筛选试验中活细胞数量的估算。我们使用MTT法评估了啮齿动物卵巢癌细胞系（DMBA-OC-1R）在暴露于顺铂和5-氟尿嘧啶的游离药物及包封（共轭和非共轭）形式组合后的活力。暴露于游离药物48小时后，可观察到明显的细胞毒性趋势，到120小时时这种趋势已得到充分证实。用含药免疫微球处理的细胞在96小时后细胞活力也出现了类似的初始下降，并持续到128小时。这些结果表明，负载化疗药物的免疫微球有潜力成功用于卵巢癌的治疗。
