One-shot velocimetry using echo planar imaging microscopy.

A rapid version of PEPI (pi-echo planar imaging) velocimetry has been implemented, enabling a velocity image, at microscopic resolution, to be acquired in less than 1 s. The velocity map was reconstructed using the phase information from the ratio of two PEPI images, one obtained with a velocity-encoding filter applied prior to the imaging sequence and the other image without. The acquisition time for each image was about 80 ms and the two complete image acquisitions were acquired in one shot in 500 ms. This rapid velocimetry sequence gave a good representation of laminar pipe flow. It has also been used to examine extensional flow in a biaxial extension in which the transient extension takes about 3 s.